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PC461 PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC461
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~53 kDa p53 protein phosphorylated at Ser15. Does not recognizes unphosphorylated p53.
Catalogue NumberPC461
Brand Family Calbiochem®
Application Data
Detection of p53, phosphorylated at Ser15 by staining paraffin sections. Sample: Human prostate carcinoma. Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb (Cat. No. PC461) (1:1000). Detection: DAB.
References
ReferencesLevine, A.J. 1997. Cell 88, 323.
Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Cho, Y., et al. 1994. Science 265, 346.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlMCF7 cells or prostate carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1:5000-1:10,000)
Paraffin Sections (1:1000-1:3000, heat pre-treatment required)
Application CommentsDoes not recognizes unphosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide [VEPPLpSQETFS(C)] corresponding to amino acids 10-20 surrounding the Ser¹⁵ phosphorylation site of human p53
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC461 0

Documentation

PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-6) Rabbit pAb Certificates of Analysis

TitleLot Number
PC461

References

Reference overview
Levine, A.J. 1997. Cell 88, 323.
Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Cho, Y., et al. 1994. Science 265, 346.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2007 RFH
ApplicationImmunoblotting (1:5000-1:10,000)
Paraffin Sections (1:1000-1:3000, heat pre-treatment required)
Application Data
Detection of p53, phosphorylated at Ser15 by staining paraffin sections. Sample: Human prostate carcinoma. Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-6) Rabbit pAb (Cat. No. PC461) (1:1000). Detection: DAB.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~53 kDa p53 phoshorylated at Ser15.
Backgroundp53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53. Multi-site phosphorylation of p53 is key in mediating many of these events. Within its N-terminal domain, p53 is phosphorylated by kinases responsive to UV radiation, cytokines, DNA damage and growth factors. C-terminal phosphorylation is mediated by protein kinases involved in growth stimulation, cell cycle control and apoptosis. These C-terminal phosphorylation events are involved in controlling the specific DNA binding function of p53, and may play a role in regulating other functions of p53.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide [VEPPLpSQETFS(C)] corresponding to amino acids 10-20 surrounding the Ser¹⁵ phosphorylation site of human p53
IsotypeIgG
Specieshuman
Positive controlMCF7 cells or prostate carcinoma tissue
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsDoes not recognizes unphosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLevine, A.J. 1997. Cell 88, 323.
Meek, D.W. 1997. Pathol. Biol. Paris 45, 804.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Cho, Y., et al. 1994. Science 265, 346.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.