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525286 PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (14B3)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
525286
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes phosphothreonine-containing proteins. Supplied with a positive control.
Catalogue Number525286
Brand Family Calbiochem®
Application Data
Detection of phosphothreonine-containing proteins by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphothreonine Mouse mAb (14B3) (Cat. No. 525286) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (1E11) (mouse) (Cat. No. 525287), and Phosphothreonine Detection Kit (Cat. No. 525288)
References
Product Information
FormLyophilized
Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2x PBS, PEG, sucrose, 10 mM NaF.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ vandate-treated A431 cells)
Application CommentsFor immunoblotting with the positive control, use 5 µl (chemiluminescence) to 20 µl (colorimetric) per mini-gel lane. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena mixture of synthetic phosphopeptides containing phosphothreonine, conjugated to KLH
Clone14B3
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml H₂O (15 min, RT). Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
525286 0

Documentation

PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (14B3) Certificates of Analysis

TitleLot Number
525286
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-February-2011 JSW
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ vandate-treated A431 cells)
Application Data
Detection of phosphothreonine-containing proteins by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphothreonine Mouse mAb (14B3) (Cat. No. 525286) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (1E11) (mouse) (Cat. No. 525287), and Phosphothreonine Detection Kit (Cat. No. 525288)
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes phosphothreonine-containing proteins. Supplied with a positive control consisting of phosphoproteins isolated from rabbit muscle by affinity chromatography using Fe3+-IDA-sepharose.
HostMouse
Immunogena mixture of synthetic phosphopeptides containing phosphothreonine, conjugated to KLH
Clone14B3
IsotypeIgG₁
Speciesa broad range of species
FormLyophilized
Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2x PBS, PEG, sucrose, 10 mM NaF.
Preservative≤0.1% sodium azide
CommentsFor immunoblotting with the positive control, use 5 µl (chemiluminescence) to 20 µl (colorimetric) per mini-gel lane. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml H₂O (15 min, RT). Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Toxicity Standard Handling