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516852 Pertussis Toxin, B Oligomer, Bordetella pertussis - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
516852
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



The pentameric cell-binding component that is responsible for binding of the holotoxin to eukaryotic cell surfaces, facilitating entry of the A protomer into receptive cells. Reported to elicit direct cellular responses by binding to several cell surface receptors with oligosaccharide side chains. Also elicits a variety of physiological responses, such as mitogenesis in human T cells, enhancement of aggregation of human platelets, elevation of cytosolic Ca2+ levels, and neutralization of antibody response in mice. Reported to deactivate CCR5 and inhibit the entry of R5 HIV-1 in activated T lymphocytes. Also blocks post-entry step of HIV-1 replication.
Catalogue Number516852
Brand Family Calbiochem®
References
ReferencesAlfano, M., et al. 2001. J. Immunol. 166, 1863.
Alfano, M., et al. 2000. J. Virol. 74, 8767.
Hazes, B., et al. 1996. J. Mol. Biol. 258, 661.
Wong, W.S., and Rosoff, P.M. 1996. Can. J. Physiol. Pharmacol. 74, 559.
Banga, S., et al. 1987. J. Biol. Chem. 262, 14871.
Burns, D.L., et al. 1987. Infect. Immun. 55, 24.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Sato, Y., et al. 1983. Infect. Immun. 41, 313.
Tamura, M., et al. 1982. Biochemistry 21, 5516.
Product Information
ATP CompetitiveN
FormLyophilized
ReversibleN
Applications
Biological Information
Primary TargetCell surface receptors with oligosaccharide side chains
PurityFour distinct bands by SDS-PAGE
Physicochemical Information
Cell permeableN
Contaminants≤1% holotoxin by CHO cell assay
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 23/24/25

Toxic by inhalation, in contact with skin and if swallowed.
S PhraseS: 45-36/37/39

In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Wear suitable protective clothing, gloves and eye/face protection.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
516852 0

Documentation

Pertussis Toxin, B Oligomer, Bordetella pertussis - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Pertussis Toxin, B Oligomer, Bordetella pertussis - Calbiochem Certificates of Analysis

TitleLot Number
516852

References

Reference overview
Alfano, M., et al. 2001. J. Immunol. 166, 1863.
Alfano, M., et al. 2000. J. Virol. 74, 8767.
Hazes, B., et al. 1996. J. Mol. Biol. 258, 661.
Wong, W.S., and Rosoff, P.M. 1996. Can. J. Physiol. Pharmacol. 74, 559.
Banga, S., et al. 1987. J. Biol. Chem. 262, 14871.
Burns, D.L., et al. 1987. Infect. Immun. 55, 24.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Sato, Y., et al. 1983. Infect. Immun. 41, 313.
Tamura, M., et al. 1982. Biochemistry 21, 5516.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-August-2008 RFH
DescriptionThe pentameric cell-binding component that is responsible for binding of the holotoxin to eukaryotic cell surfaces, facilitating entry of the A protomer into receptive cells. Reported to elicit direct cellular responses by binding to several cell surface receptors with oligosaccharide side chains. Also causes a variety of physiological responses, such as mitogenesis in human T-cells, enhancement of aggregation of human platelets, elevation of cytosolic Ca2+ levels, and neutralization of antibody response in mice.
FormLyophilized
PurityFour distinct bands by SDS-PAGE
Contaminants≤1% holotoxin by CHO cell assay
SolubilitySterile distilled H₂O or aqueous buffers
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.
Toxicity Toxic
ReferencesAlfano, M., et al. 2001. J. Immunol. 166, 1863.
Alfano, M., et al. 2000. J. Virol. 74, 8767.
Hazes, B., et al. 1996. J. Mol. Biol. 258, 661.
Wong, W.S., and Rosoff, P.M. 1996. Can. J. Physiol. Pharmacol. 74, 559.
Banga, S., et al. 1987. J. Biol. Chem. 262, 14871.
Burns, D.L., et al. 1987. Infect. Immun. 55, 24.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Sato, Y., et al. 1983. Infect. Immun. 41, 313.
Tamura, M., et al. 1982. Biochemistry 21, 5516.

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Categories

Life Science Research > Inhibitors and Biochemicals > Biochemicals > Toxins