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512743 Pasteurella Multocida Toxin, Pasteurella multocida - CAS 129876-91-1 - Calbiochem

A bacterial protein toxin that binds to and enters eukaryotic cells via receptor-mediated endocytosis.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
512743
  
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Overview

Replacement Information

Key Spec Table

CAS #
129876-91-1
Description
Overview

This product has been discontinued.



A bacterial protein toxin that binds to and enters eukaryotic cells via receptor-mediated endocytosis. Acts intracellularly to initiate the inositol trisphosphate signaling pathway, Ca2+ mobilization, and DNA synthesis. Stimulates tyrosine phosphorylation of multiple substrates including focal adhesion kinase. Exerts its action on the α-subunit of the Gq family of G-proteins. Has been used for the activation of Gq-protein-coupled phosphatidylinositol-specific phospholipase C (PLC) in intact cells, making PMT useful as a tool for studying Gq-linked signal transduction.
Catalogue Number512743
Brand Family Calbiochem®
References
ReferencesSeo, B., et al. 2000. J. Biol. Chem. 275, 2239.
Essler, M., et al. 1998. J. Immunol. 161, 5640.
Wilson, B.A., et al. 1997. J. Biol. Chem. 272, 1268.
Lacerda, H.M., et al. 1996. J. Biol. Chem. 271, 439.
Foged, N.T. 1992. Acta Pathol. Microbiol. Immunol. Scand. 100 (Suppl. 25), 1.
Staddon, J.M., et al. 1991. J. Biol. Chem. 266, 4840.
Buys, W.E., et al. 1990. Nucleic Acids Res. 18, 2815.
Lax, A.J., et al. 1990. FEBS Lett. 227, 59.
Petersen, S.K. 1990. Mol. Microbiol. 4, 821.
Foged, N.T., et al. 1987. FEMS Microbiol. Lett. 43, 45.
Product Information
CAS number129876-91-1
ATP CompetitiveN
FormLyophilized solid
FormulationLyophilized from 100 mM NaCl, 30 mM Tris, 0.1% sucrose, pH 8.3.
ReversibleN
Applications
Biological Information
Primary TargetActs intracellularly to initiate the inositol trisphosphate signaling pathway, Ca2+ mobilization, and DNA synthesis
PuritySingle band by SDS-PAGE
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 23/24/25

Toxic by inhalation, in contact with skin and if swallowed.
S PhraseS: 36/37/39-45

Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
512743 0

Documentation

Pasteurella Multocida Toxin, Pasteurella multocida - CAS 129876-91-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Pasteurella Multocida Toxin, Pasteurella multocida - CAS 129876-91-1 - Calbiochem Certificates of Analysis

TitleLot Number
512743

References

Reference overview
Seo, B., et al. 2000. J. Biol. Chem. 275, 2239.
Essler, M., et al. 1998. J. Immunol. 161, 5640.
Wilson, B.A., et al. 1997. J. Biol. Chem. 272, 1268.
Lacerda, H.M., et al. 1996. J. Biol. Chem. 271, 439.
Foged, N.T. 1992. Acta Pathol. Microbiol. Immunol. Scand. 100 (Suppl. 25), 1.
Staddon, J.M., et al. 1991. J. Biol. Chem. 266, 4840.
Buys, W.E., et al. 1990. Nucleic Acids Res. 18, 2815.
Lax, A.J., et al. 1990. FEBS Lett. 227, 59.
Petersen, S.K. 1990. Mol. Microbiol. 4, 821.
Foged, N.T., et al. 1987. FEMS Microbiol. Lett. 43, 45.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-August-2008 RFH
DescriptionA dermatonecrotic toxin produced by some strains of Pasteurella multocida. PMT binds to and enters mammalian cells via a receptor-mediated endocytosis mechanism that is not well defined. Causes a transient activation of G-protein, Gqα. Activates phospholipase C and stimulates the formation of inositol phospholipids and diacylglycerol that results in Ca2+ mobilization from an intracellular pool and translocation of protein kinase C to cell membranes. PMT is also reported to stimulate tyrosine phosphorylation of focal adhesion kinase (FAK125) and paxillin. Reported to activate stress fiber formation and increase trans-endothelial permeability by stimulating Rho kinase and myosin light chain phosphatase.
FormLyophilized solid
FormulationLyophilized from 100 mM NaCl, 30 mM Tris, 0.1% sucrose, pH 8.3.
CAS number129876-91-1
PuritySingle band by SDS-PAGE
SolubilityReconstitute in 500 µl sterile distilled H₂O
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Toxicity Toxic
ReferencesSeo, B., et al. 2000. J. Biol. Chem. 275, 2239.
Essler, M., et al. 1998. J. Immunol. 161, 5640.
Wilson, B.A., et al. 1997. J. Biol. Chem. 272, 1268.
Lacerda, H.M., et al. 1996. J. Biol. Chem. 271, 439.
Foged, N.T. 1992. Acta Pathol. Microbiol. Immunol. Scand. 100 (Suppl. 25), 1.
Staddon, J.M., et al. 1991. J. Biol. Chem. 266, 4840.
Buys, W.E., et al. 1990. Nucleic Acids Res. 18, 2815.
Lax, A.J., et al. 1990. FEBS Lett. 227, 59.
Petersen, S.K. 1990. Mol. Microbiol. 4, 821.
Foged, N.T., et al. 1987. FEMS Microbiol. Lett. 43, 45.