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14-621M PTP-1B Protein, 250 µg

N-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321, for use in Phosphatase Assays.

14-621M
250 µg  Also available in 10 μg size (cat#14-621) and in bulk (cat#14-621-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-621M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPTP-1B Protein, 250 µg
OverviewN-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321
References
Product Information
Quality LevelMQ300
Applications
ApplicationN-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321, for use in Phosphatase Assays.
Key Applications
  • Phosphatase Assay
Biological Information
Sourcehuman PTP-1B expressed in E. coli
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Gene Symbol
  • PTPN1
  • PTP1B
  • PTP-1B
Protein TargetPTP-1B
Purification MethodGlutathione-agarose
Target Sub-FamilyTyr phosphatase
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
SIZE: 435 amino acids; 49967 Da
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
Molecular Weight64.1kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by the dephosphorylation of 100μM DiFMUP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-621) and in bulk (cat#14-621-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-621M 04055977311259

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-621 PTP-1B Protein, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
Life Science Research > Proteins and Enzymes > Purified Phosphatases