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14-844M PIP5K1α Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant human PIP5K1α, full length. For use in Kinase Assays.

PIP5K1α Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-844M
250 µg  Also available in 10 μg size (cat#14-844) and in bulk (cat# 14-844-K).
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-844M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPIP5K1α Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant human PIP5K1α, full length.
Product Information
Presentation10µg of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant human PIP5K1α, full length. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryParticipates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2 (By similarity)
Gene Symbol
  • PIP5K1A
Protein TargetPIP5K1α
Purification MethodImmobilized metal affinity chromatography (IMAC)
Target Sub-FamilyPIPK
UniProt Number
UniProt SummaryFUNCTION: Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
SUBUNIT STRUCTURE: Interacts with ARF1, RAC1, PLD1, PLD2 and JUB
SUBCELLULAR LOCATION: Cell membrane. Endomembrane system. Golgi apparatus › Golgi stack. Note: Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks. Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
SIMILARITY: Contains 1 PIPK domain.
Molecular Weight60kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated in the PIProfiler.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-844) and in bulk (cat# 14-844-K).
Global Trade Item Number
Catalogue Number GTIN
14-844M 04053252669231