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14-901 PIP4K2α Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant human PIP4K2α, full length. For use in Kinase Assays.

PIP4K2α Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-901
10 µg  Also available in 250μg size as catalog # 14-901M - Call for pricing and availability.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-901
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPIP4K2α Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant human PIP4K2α, full length.
References
Product Information
Presentation10µg of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant human PIP4K2α, full length. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
Gene Symbol
  • PIP4K2A
  • PIP5K2
  • PIP5K2A
Protein TargetPIP4K2α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPIPK
UniProt Number
UniProt SummaryFUNCTION: Catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
SUBUNIT STRUCTURE: Homodimer
TISSUE SPECIFICITY: Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
SIMILARITY: Contains 1 PIPK domain.
Molecular Weight51kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in the PIProfiler.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-901M - Call for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-901 04053252402692

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Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases