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71003 NovaTaq™ DNA Polymerase

Ultrapure recombinant enzyme for dependable PCR amplification

NovaTaq™ DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71003
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71003-3 Glass bottle 100 u
71003-4 Glass bottle 500 u
71003-5 Glass bottle 2500 u
Description
OverviewNovaTaq™ DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.
Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration 5.0 U/µl
Purity > 95% homogeneous by SDS-PAGE
Endonuclease None detected
Amplification effiency Functional PCR
Storage –20°C

Catalogue Number71003 Brand Family Novagen®

References
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-<Sup>32</Sup>P]dCTP, and activated salmon sperm DNA.
•100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase
•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl₂
•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer without MgCl₂
•1 or 2 or 7 × 1.5 ml25 mM MgCl₂
DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71003-3 04055977255645
71003-4 04055977255652
71003-5 04055977255669