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492002 NOX1 Inhibitor, ML171 - CAS 6631-94-3 - Calbiochem

The NOX1 Inhibitor, ML171, also referenced under CAS 6631-94-3, controls the biological activity of NOX1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NADPH Oxidase-1 Inhibitor, ML171, 2-APT, 2-acetylphenothiazine

492002
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
6631-94-3C₁₄H₁₁NOS

Products

Catalogue NumberPackaging Qty/Pack
492002-10MG Glass bottle 10 mg
Description
OverviewA highly selective, cell-permeable, and reversible 2-acetylphenothiazine that is shown to inhibit NADPH Oxidase-1 (Nox1) (IC50 = 0.129 µM, and 0.25 µM) in human HT29 and HEK293 cell-based assays, respectively. Unlike most other currently used Nox inhibitors, it only has marginal activity on other cellular ROS-producing enzymes and receptors including the other Nox isoforms (IC50 = 5 µM, 3 µM and 5 µM for Nox2, Nox3, and Nox4, respectively) and xanthine oxidase, XO, (IC50 = 5.5 µM) in HEK293 cell-based assays. It is also shown to block Nox1-dependent ROS generation in HEK293 cultures dose-dependently at concentrations up to ~ 100 nM; however, its inhibitory effect is reversed through over-expression of Nox1, thereby suggesting that this compound is highly selective for Nox1, with little effect against Nox2-dependent ROS generation (IC50 > 10 µM). Qualitatively, it displays an inhibitory effect against SrcYF-induced ECM-degrading invadopodia formation in DLD1 Human Colon Cancer Cells.

Note: this compound has been reported to interfere with peroxidase-dependent ROS-measuring assays and show no effect on PMA-induced respiratory burst of human granulocytes (Seredenina, T., et al).
Catalogue Number492002
Brand Family Calbiochem®
SynonymsNADPH Oxidase-1 Inhibitor, ML171, 2-APT, 2-acetylphenothiazine
References
ReferencesSeredenina, T., et al. 2015. Free Radic. Biol. Med. 239.
Gianni, D., et al. 2010. ACS Chem. Biol. 5, 981.
Product Information
CAS number6631-94-3
FormDark yellow-orange powder
Hill FormulaC₁₄H₁₁NOS
Chemical formulaC₁₄H₁₁NOS
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
492002-10MG 04055977273465

Documentation

NOX1 Inhibitor, ML171 - CAS 6631-94-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NOX1 Inhibitor, ML171 - CAS 6631-94-3 - Calbiochem Certificates of Analysis

TitleLot Number
492002

References

Reference overview
Seredenina, T., et al. 2015. Free Radic. Biol. Med. 239.
Gianni, D., et al. 2010. ACS Chem. Biol. 5, 981.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-November-2020 JSW
SynonymsNADPH Oxidase-1 Inhibitor, ML171, 2-APT, 2-acetylphenothiazine
DescriptionA highly selective, cell-permeable, and reversible 2-acetylphenothiazine that is shown to inhibit NADPH Oxidase-1 (Nox1) (IC50 = 0.129 µM, and 0.25 µM) in human HT29 and HEK293 cell-based assays, respectively. Unlike most other currently used Nox inhibitors, it only has marginal activity on other cellular ROS-producing enzymes and receptors including the other Nox isoforms (IC50 = 5 µM, 3 µM and 5 µM for Nox2, Nox3, and Nox4, respectively) and xanthine oxidase, XO, (IC50 = 5.5 µM) in HEK293 cell-based assays. It is also shown to block Nox1-dependent ROS generation in HEK293 cultures dose-dependently at concentrations up to ~100 nM; however, its inhibitory effect is reversed through over-expression of Nox1, thereby suggesting that this compound is highly selective for Nox1, with little effect against Nox2-dependent ROS generation (IC50 > 10 µM). Qualitatively, it displays an inhibitory effect against SrcYF-induced ECM-degrading invadopodia formation in DLD1 Human Colon Cancer Cells.

Note: this compound has been reported to interfere with peroxidase-dependent ROS-measuring assays and show no effect on PMA-induced respiratory burst of human granulocytes (Seredenina, T., et al).
FormDark yellow-orange powder
Intert gas (Yes/No) Packaged under inert gas
CAS number6631-94-3
Chemical formulaC₁₄H₁₁NOS
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Regulatory Review
ReferencesSeredenina, T., et al. 2015. Free Radic. Biol. Med. 239.
Gianni, D., et al. 2010. ACS Chem. Biol. 5, 981.