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482251 NIPP-1, His•Tag®, Bovine Thymus, Recombinant, E. coli - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Nuclear Inhibitor of Protein Phosphatase-1

Primary Target: PP1
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Overview

Description
OverviewRecombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor of protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases including PP2A, PP2B, and PP2C. Also an ideal substrate for protein kinase A and casein kinase II. Thought to be involved in the targeting of PP1 to RNA-associated substrates and may also be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor Rb.

This product has been discontinued.
Catalogue Number482251
Brand Family Calbiochem®
SynonymsNuclear Inhibitor of Protein Phosphatase-1
References
ReferencesBeullens, M., et al. 1999. J. Biol. Chem. 274, 14053.
Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067.
Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257.
Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068.
Van Eynde, A., et al. 1994. Biochem. J. 297, 447.
Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172.
Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538.
Product Information
ATP CompetitiveN
FormLiquid
FormulationIn 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5.
ReversibleN
Biological Information
Primary TargetPP1
Primary Target K<sub>i</sub>1-10 pM against protein phosphatase 1 (PP1)
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
482251 0