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616387 Nα-Tosyl-Phe Chloromethyl Ketone

Irreversible inhibitor of chymotrypsin.

Nα-Tosyl-Phe Chloromethyl Ketone MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TPCK

Primary Target: chymotrypsin activity in preparations
616387
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
402-71-1C₁₇H₁₈ClNO₃S

Products

Catalogue NumberPackaging Qty/Pack
616387-250MG Plastic ampoule 250 mg
Description
Overview

This product has been discontinued.





Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides (EC50 = 20 µM).
Catalogue Number616387
Brand Family Calbiochem®
SynonymsTPCK
References
ReferencesFearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Murakami, T., et al. 1991. Biochim. Biophys. Acta 1079, 279.
Nierodzik, M.L., et al. 1991. J. Clin. Invest. 87, 229.
Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.
Product Information
CAS number402-71-1
ATP CompetitiveN
FormWhite solid
Hill FormulaC₁₇H₁₈ClNO₃S
Chemical formulaC₁₇H₁₈ClNO₃S
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetchymotrypsin activity in preparations
Primary Target IC<sub>50</sub>EC50 = 20 µM in blocking the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides.
Purity≥98% by TLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXT5613500
Safety Information
R PhraseR: 41

Risk of serious damage to eyes.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 4 months at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616387-250MG 04055977186314

Documentation

Nα-Tosyl-Phe Chloromethyl Ketone SDS

Title

Safety Data Sheet (SDS) 

Nα-Tosyl-Phe Chloromethyl Ketone Certificates of Analysis

TitleLot Number
616387

References

Reference overview
Fearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
Murakami, T., et al. 1991. Biochim. Biophys. Acta 1079, 279.
Nierodzik, M.L., et al. 1991. J. Clin. Invest. 87, 229.
Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.

Citations

Title
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-September-2008 RFH
    SynonymsTPCK
    DescriptionIrreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharide (EC50 = 20 µM). Suggested working concentration is 10-100 µM.
    FormWhite solid
    CAS number402-71-1
    RTECSXT5613500
    Chemical formulaC₁₇H₁₈ClNO₃S
    Structure formulaStructure formula
    Purity≥98% by TLC
    SolubilityEthanol (5 mg/ml) or Methanol (5 mg/ml)
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 4 months at 4°C.
    Toxicity Irritant
    ReferencesFearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
    Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
    Weaver, V.M., et al. 1993. Biochem. Cell Biol. 71, 488.
    Murakami, T., et al. 1991. Biochim. Biophys. Acta 1079, 279.
    Nierodzik, M.L., et al. 1991. J. Clin. Invest. 87, 229.
    Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.
    Citation
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    		•