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616382 Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem

Inhibits trypsin-like serine proteinases.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TLCK

Primary Target: trypsin like proteases
616382
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4238-41-9C₁₄H₂₁ClN₂O₃S . HCl

Products

Catalogue NumberPackaging Qty/Pack
616382-250MG Plastic ampoule 250 mg
616382-50MG Plastic ampoule 50 mg
Description
OverviewInhibits trypsin-like serine proteinases. Irreversibly inactivates trypsin without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to the target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemia cells and normal thymocytes.
Catalogue Number616382
Brand Family Calbiochem®
SynonymsTLCK
References
ReferencesGriscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
Kim, H., et al. 1995. J. Immunol. 154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
Bruno, S., et al. 1992. Leukemia 6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.
Product Information
CAS number4238-41-9
ATP CompetitiveN
FormOff-white solid
Hill FormulaC₁₄H₂₁ClN₂O₃S . HCl
Chemical formulaC₁₄H₂₁ClN₂O₃S . HCl
Hygroscopic Hygroscopic
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targettrypsin like proteases
Primary Target IC<sub>50</sub>EC50 = 80 µM blocking nitric oxide synthase induced by interferon-γ and lipopolysaccharides in macrophages
Purity≥95% by elemental analysis
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXT5160000
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsUnstable in solution; reconstitute just prior to use.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616382-250MG 04055977186284
616382-50MG 04055977186291

Documentation

Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride - CAS 4238-41-9 - Calbiochem Certificates of Analysis

TitleLot Number
616382

References

Reference overview
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
Kim, H., et al. 1995. J. Immunol. 154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
Bruno, S., et al. 1992. Leukemia 6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.

Citations

Title
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision18-September-2008 JSW
    SynonymsTLCK
    DescriptionInhibits trypsin-like serine proteases. Inactivates trypsin irreversibly without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Suggesting working concentration is 10-100 µM.
    FormOff-white solid
    CAS number4238-41-9
    RTECSXT5160000
    Chemical formulaC₁₄H₂₁ClN₂O₃S . HCl
    Structure formulaStructure formula
    Purity≥95% by elemental analysis
    SolubilityAqueous solutions pH <6.0 (1 mg/ml)
    Storage +2°C to +8°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsUnstable in solution; reconstitute just prior to use.
    Toxicity Irritant
    ReferencesGriscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun. 215, 721.
    Kim, H., et al. 1995. J. Immunol. 154, 4741.
    Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
    Biro, A., et al. 1992. Eur. J. Immunol. 22, 2547.
    Bruno, S., et al. 1992. Leukemia 6, 1113.
    Lee, S.F. 1992. Infect. Immun. 60, 4032.
    Schmidt, H.H., et al. 1992. Mol. Pharmacol. 41, 615.
    Citation
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
    		•