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411452 Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
411452
  
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Overview

Replacement Information
Description
Catalogue Number411452
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Product Information
FormLiquid
FormulationUndiluted ascites.
PreservativeNone
Applications
Application ReferencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Key Applications Enzyme Immunoassay
Application NotesELISA (1:2000-1:10,000, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: <0.4%
Human IgG3: <0.4%
Human IgG4: <0.05%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: <0.02%
Human IgG3: <0.02%
Human IgG4: <0.02%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.02%
Biological Information
ImmunogenHuman
CloneHP6069
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
411452 0

Documentation

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Certificates of Analysis

TitleLot Number
411452

References

Reference overview
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationELISA (1:2000-1:10,000, see application references)
DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes human IgG1 Fc fragment.
BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
HostMouse
Immunogen speciesHuman
CloneHP6069
IsotypeIgG₁
FormLiquid
FormulationUndiluted ascites.
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: <0.4%
Human IgG3: <0.4%
Human IgG4: <0.05%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: <0.02%
Human IgG3: <0.02%
Human IgG4: <0.02%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.02%
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
Application referencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.