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CBL496-25UG Mouse Anti-Human CD34 Monoclonal Antibody

Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).

Mouse Anti-Human CD34 Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD34 UniProt Number: P28906
CBL496-25UG
25 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MkFC, IP, IHC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL496-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionMouse Anti-Human CD34 Monoclonal Antibody
References
Product Information
FormatPurified
PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.
Quality LevelMQ100
Applications
ApplicationAnti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
Application NotesIdentification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman endothelial vesicles
CloneQBEND/10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThe antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.

FUSION PARTNER: NSO myeloma cell line
IsotypeIgG1
Species Reactivity
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
Gene Symbol
  • CD34
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in the United States.
Quality AssuranceTested
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL496-25UG 04054839350498