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530390 Mitosis Inhibitor, AK301 - Calbiochem

A cell permeable compound that irreversibly arrests mitosis in colon cancer cells (IC₅₀ = 115 nM in HT29 cells).

Mitosis Inhibitor, AK301 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TNF sensitizing agent

Primary Target: tubulin
530390
  
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₉H₂₁ClN₂O₂
Description
Overview

This product has been discontinued.



A cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
Catalogue Number530390
Brand Family Calbiochem®
SynonymsTNF sensitizing agent
References
ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
Product Information
FormWhite solid
Hill FormulaC₁₉H₂₁ClN₂O₂
Chemical formulaC₁₉H₂₁ClN₂O₂
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary Targettubulin
Primary Target IC<sub>50</sub>115 nM
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
530390 0

Documentation

Mitosis Inhibitor, AK301 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Chopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-May-2014 JSW
SynonymsTNF sensitizing agent
DescriptionA cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₉H₂₁ClN₂O₂
Purity≥98% by HPLC
SolubilityDMSO (25 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.