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445885 Metastin, Synthetic

A KiSS-1 gene product with 54 amino-acid residues and a C-terminally amidated LRF-motif that acts as a metastasis inhibitor via G-protein-coupled receptors (GPCRs).

Metastin, Synthetic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: KiSS-1 (68-121), Kisspeptin-54

Primary Target: G-protein-coupled receptors (GPCRs)
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₅₈H₄₀₁N₇₉O₇₈
Description
Overview

This product has been discontinued.





A KiSS-1 gene product with 54 amino-acid residues and a C-terminally amidated LRF-motif that acts as a metastasis inhibitor via G-protein-coupled receptors (GPCRs). Reported to inhibit chemotaxis and invasion of hOT7T175-transfected CHO cells in vitro (IC50 = 50 nM) and attenuates pulmonary metastasis of hOT7T175-transfected B16-BL6 melanomas in vivo. Shown to be a potent and selective agonist of AXOR12 and hOT7T175 (a novel human GPCR). Induces an increase in intracellular Ca2+ levels in CHO/h175 and CHO/AXOR12:Gqi5 cells. Binds to hOT7T175 with high affinity (Ki = 340 pM, determined by competitive binding analysis).
Catalogue Number445885
Brand Family Calbiochem®
SynonymsKiSS-1 (68-121), Kisspeptin-54
References
ReferencesKotani, M., et al. 2001. J. Biol. Chem. 276, 34631.
Ohtaki, T., et al. 2001. Nature 411, 613.
Muir, A.I., et al. 2001. J. Biol. Chem. 276, 28969.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₂₅₈H₄₀₁N₇₉O₇₈
Chemical formulaC₂₅₈H₄₀₁N₇₉O₇₈
Hygroscopic Hygroscopic
ReversibleN
Applications
Biological Information
Primary TargetG-protein-coupled receptors (GPCRs)
Primary Target IC<sub>50</sub>50 nM inhibiting chemotaxis and invasion of hOT7T175-transfected CHO cells in vitro
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceH-Gly-Thr-Ser-Leu-Ser-Pro-Pro-Pro-Glu-Ser-Ser-Gly-Ser-Arg-Gln-Gln-Pro-Gly-Leu-Ser-Ala-Pro-His-Ser-Arg-Gln-Ile-Pro-Ala-Pro-Gln-Gly-Ala-Val-Leu-Val-Gln-Arg-Glu-Lys-Asp-Leu-Pro-Asn-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
445885 0

Documentation

Metastin, Synthetic Certificates of Analysis

TitleLot Number
445885

References

Reference overview
Kotani, M., et al. 2001. J. Biol. Chem. 276, 34631.
Ohtaki, T., et al. 2001. Nature 411, 613.
Muir, A.I., et al. 2001. J. Biol. Chem. 276, 28969.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2008 RFH
SynonymsKiSS-1 (68-121), Kisspeptin-54
DescriptionA KiSS-1 gene product with 54 amino acids and a C-terminally amidated LRF-motif that acts as a metastasis inhibitor via G-protein-coupled receptors (GCPRs). Reported to inhibit chemotaxis and invasion of hOT7T175-transfected CHO cells in vitro (IC50 = 50 nM) and attenuates pulmonary metastasis of hOT7T175-transfected B16-BL6 melanomas in vivo. Shown to be a potent and selective agonist of AXOR12 and hOT7T175 (a novel human GPCR). Induces an increase in intracellular Ca2+ levels in CHO/h175 and CHO/AXOR12:Gqi5 cells. Binds to hOT7T175 with high affinity (Ki = 340 pM, determined by competitive binding analysis). Has also been shown to be a ligand for GPR54, an orphan G protein-coupled receptor.
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₅₈H₄₀₁N₇₉O₇₈
Peptide SequenceH-Gly-Thr-Ser-Leu-Ser-Pro-Pro-Pro-Glu-Ser-Ser-Gly-Ser-Arg-Gln-Gln-Pro-Gly-Leu-Ser-Ala-Pro-His-Ser-Arg-Gln-Ile-Pro-Ala-Pro-Gln-Gly-Ala-Val-Leu-Val-Gln-Arg-Glu-Lys-Asp-Leu-Pro-Asn-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesKotani, M., et al. 2001. J. Biol. Chem. 276, 34631.
Ohtaki, T., et al. 2001. Nature 411, 613.
Muir, A.I., et al. 2001. J. Biol. Chem. 276, 28969.