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234378 Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

234378
  
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Overview

Replacement Information
Description
OverviewRecombinant, mouse macrophage colony-stimulating factor expressed in E. coli. Stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
Catalogue Number234378
Brand Family Calbiochem®
SynonymsrmM-CSF
References
ReferencesBergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 6706.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg M-CSF.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/ug M-CSF.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSGI5500000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze(-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
234378 0

Documentation

Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
234378

References

Reference overview
Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 6706.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-June-2008 RFH
SynonymsrmM-CSF
DescriptionStimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg M-CSF.
RTECSGI5500000
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/ug M-CSF.
Biological activityED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze(-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Toxicity Standard Handling
ReferencesBergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 6706.