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SCYT1-91K MILLIPLEX® Ovine Cytokine/Chemokine Panel 1 - Immunology Multiplex Assay

The Ovine Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 14 cytokine and chemokine biomarkers in ovine serum, plasma and cell culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms, Inflammation, Infectious Diseases, Cytokines & Cytokine Receptors, Oncology, Obesity, Metabolic Disorders
SCYT1-91K
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Overview

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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
IFN-γ IL-1α IL-1β IL-4 IL-6 IL-10 IL-8 IL-17A MIP-1α IL-36RA IP-10 MIP-1β TNFα VEGF-A ShMplexNote: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.Luminex xMAP
Description
Catalogue NumberSCYT1-91K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Ovine Cytokine/Chemokine Panel 1 - Immunology Multiplex Assay
OverviewMILLIPLEX® Ovine Cytokine/Chemokine Panel 1 may be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture supernatant samples: IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-17A, IL-36RA (IL-1F5), IP-10 (CXCL10), MIP-1α (CCL3), MIP-1β (CCL4), TNFα, VEGF-A. Note: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.

MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

The MILLIPLEX® Ovine Cytokine/Chemokine Panel 1 thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression.
Background InformationInflammation and immune cell response are key to maintaining homeostasis. Inflammation plays a central role in the etiology of many immunologically related diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokines in sheep samples has been limited primarily to ELISAs. Researchers now have the ability to measure multiple inflammation biomarkers simultaneously using our MILLIPLEX® Ovine Cytokine/Chemokine Panel 1, based on the Luminex® xMAP® technology.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <20%
Multiplex Ordering RequirementsNote: The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationThe Ovine Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 14 cytokine and chemokine biomarkers in ovine serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay.
• The protocol recommends a 1:2 dilution of serum and plasma samples, except when assaying IL-8 (CXCL8) in serum samples for which an up to 1:8 dilution of sample may be required.
• This kit may be used for the analysis of any or all of the Ovine Cytokine/Chemokine Panel 1 analytes in ovine tissue/cell lysate and culture supernatant samples and serum or plasma samples.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Sheep
Analytes Available
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-4
  • IL-6
  • IL-10
  • IL-8
  • IL-17A
  • MIP-1α
  • IL-36RA
  • IP-10
  • MIP-1β
  • TNFα
  • VEGF-A
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 83% - 115%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines and chemokines.
Standard Curve Range
  • IL-1α: 0.19 - 3,000 pg/mL
  • IFNγ: 0.32 - 5,000 pg/mL
  • IL-36RA: 0.35 - 5,500 pg/mL
  • IL-8, IP-10, VEGF-A: 0.77 - 12,000 pg/mL
  • IL-10, IL-17A: 1.6 - 25,000 pg/mL
  • MIP-1β: 3.8 - 60,000 g/mL
  • IL-4: 6.4 - 100,000 pg/mL
  • IL-6, MIP-1α: 7.7 - 120,000 pg/mL
  • IL-1β: 12.8 - 200,000 pg/mL
  • TNFα: 48 - 750,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCYT1-91K 04061842306893