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444155 MDMX Antagonist, SJ-172550 - CAS 431979-47-4 - Calbiochem

The MDMX Antagonist, SJ-172550, also referenced under CAS 431979-47-4, controls the biological activity of MDMX.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Methyl-2-(2-chloro-6-ethoxy-4-(E)-(3-methyl-5-oxo-1-phenyl-pyrazol-4-ylidene)methyl)phenoxy)acetate, MDMX Inhibitor II

444155
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
431979-47-4C₂₂H₂₁ClN₂O₅
Description
OverviewA cell-permeable phenyl-pyrazolylidene compound that competes with p53-derived peptide (aa 15-29) binding to MDMX in cell-free binding assays (EC50 = 2.3 µM) in a reversible manner and effectively disrupts cellular p53-MDMX interaction (IC50 <20 µM in C33A, HER, and Weri1 cultures), while exhibiting much weaker affinity toward MDM2 (EC50 = 26 µM in p53 peptide competition assays). MDMX inhibition by 20 µM SJ-172550 and MDM2 inhibition by 5 µM Nutlin-3a (Cat. Nos. 444143 & 444151) are both shown to result in reduced proliferation and increased cellular caspase-3 in cultured cells, however, only MDM2, but not MDMX, inhibition results in a significant p53 protein level upregulation.
Catalogue Number444155
Brand Family Calbiochem®
SynonymsMethyl-2-(2-chloro-6-ethoxy-4-(E)-(3-methyl-5-oxo-1-phenyl-pyrazol-4-ylidene)methyl)phenoxy)acetate, MDMX Inhibitor II
References
ReferencesReed, D., et al. 2010. J. Biol. Chem. 285, 10786.
Product Information
CAS number431979-47-4
FormOrange solid
Hill FormulaC₂₂H₂₁ClN₂O₅
Chemical formulaC₂₂H₂₁ClN₂O₅
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by NMR
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444155 0

Documentation

MDMX Antagonist, SJ-172550 - CAS 431979-47-4 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MDMX Antagonist, SJ-172550 - CAS 431979-47-4 - Calbiochem Certificates of Analysis

TitleLot Number
444155

References

Reference overview
Reed, D., et al. 2010. J. Biol. Chem. 285, 10786.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-September-2012 JSW
SynonymsMethyl-2-(2-chloro-6-ethoxy-4-(E)-(3-methyl-5-oxo-1-phenyl-pyrazol-4-ylidene)methyl)phenoxy)acetate, MDMX Inhibitor II
DescriptionA cell-permeable phenyl-pyraz-olylidene compound that competes with p53-derived peptide (aa 15-29) binding to MDMX in cell-free binding assays (EC50 = 2.3 µM) in a reversible manner and effectively disrupts cellular p53-MDMX interaction (IC50 <20 µM in C33A, HER, and Weri1 cultures), while exhibiting much weaker affinity toward MDM2 (EC50 = 26 µM in p53 peptide competition assays). MDMX inhibition by 20 µM SJ-172550 and MDM2 inhibition by 5 µM Nutlin-3a (Cat. Nos. 444143 & 444151) are both shown to result in reduced proliferation and increased cellular caspase-3 in cultured cells, however, only MDM2, but not MDMX, inhibition results in a significant p53 protein level upregulation.
FormOrange solid
Intert gas (Yes/No) Packaged under inert gas
CAS number431979-47-4
Chemical formulaC₂₂H₂₁ClN₂O₅
Structure formulaStructure formula
Purity≥95% by NMR
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesReed, D., et al. 2010. J. Biol. Chem. 285, 10786.