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105387 Leishman's eosin methylene blue solution modified

for microscopy

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
105387
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1053870500 Plastic bottle 500 ml
Description
Catalogue Number105387
DescriptionLeishman's eosin methylene blue solution modified
OverviewThe staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright's eosin methylene blue solution and May-Grünwald's eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
A 500 mL bottle of Leishman's eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells.As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Boiling point65 °C (1013 hPa)
Density0.79 g/cm3 (20 °C)
Explosion limit5.5 - 44 %(V) Methanol
Flash point12 °C
Ignition temperature455 °C Methanol
Vapor pressure128 hPa (20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
H317: May cause an allergic skin reaction.
H370: Causes damage to organs.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal1
Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of dangerhighly flammable, toxic
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
Supplemental Information
Specifications
Suitability for microscopy (Blood smear)passes test
Erythrocytesreddish
Nucleiviolet
Eosinophilic granulesbrick-red
Neutrophilic granuleslight violet
Basophilic granulesdark violet
Lymphocyte cytoplasmnblue
Monocyte cytoplasmgrey-blue
Thrombocytesviolet
Global Trade Item Number
Catalogue Number GTIN
1053870500 04022536063911

Documentation

Leishman's eosin methylene blue solution modified SDS

Title

Safety Data Sheet (SDS) 

Leishman's eosin methylene blue solution modified Certificates of Analysis

Product Number PackagingSpecificationLot Number
1053870500 Plastic bottlePDF Specification Document

Related Products & Applications

Alternative Products

Catalogue Number Description
101350 Leishman's eosine methylene blue

Accessories (Primary)

Catalogue Number Description
111374 Buffer tablets pH 6.8
106009 Methanol
109468 Buffer tablets pH 7.2

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Categories

IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions