Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

407631 Interleukin-3, Mouse, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rmIL-3

407631
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, mouse interleukin-3 expressed in E. coli. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils.
Catalogue Number407631
Brand Family Calbiochem®
SynonymsrmIL-3
References
ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407631 0

Documentation

Interleukin-3, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Interleukin-3, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407631

References

Reference overview
Muther, H., et al. 1994. Growth Factors 10, 17.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 RFH
SynonymsrmIL-3
DescriptionRecombinant, mouse interleukin-3 expressed in E. coli. IL-3 is a hemopoietic colony stimulating factor that interacts with very early multipotent hematopoietic progenitor cells. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils. IL-3 supports colony formation in the soft agar of bone marrow cells of granulocyte, macrophage, megakaryocyte, and erythrocyte lineages. Unlike human IL-3, the murine form supports the growth of mast cells. IL-3 activity is highly species-specific. Murine IL-3 does not show activity on human cells. Recombinant IL-3 produced in and purified from E. coli.
FormLyophilized
FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
SolubilitydH₂O. Reconstitute to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low-endotoxin medium or buffer containing FBS or tissue culture grade BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
Merck USA index14, 4995
ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.