Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IF002 Interferon-γ Protein, Recombinant human

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

Interferon-γ Protein, Recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P01579 Entrez Gene Number: NM_000619.2
IF002
100 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman Human, expressed in E.coliGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
Description
Catalogue NumberIF002
ReplacesIF3
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionInterferon-γ Protein, Recombinant human
OverviewInterferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
References
Product Information
HS Code3502 90 90
PresentationSterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Quality LevelMQ100
Applications
ApplicationThe Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.
Key Applications
  • Cell Culture
Application NotesFor most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
SourceHuman, expressed in E.coli
Specific ActivityHuman IFN-gamma is fully biologically active when compared to standards. The ED(50) as determined in a viral resistance assay is <= 0.05 ng/mL corresponding to a specific activity of >= 2 x 10(E7) units/mg.
Entrez Gene Number
Gene Symbol
  • IFNG
  • IFN-gamma
  • IFI
  • IFG
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01579 # Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
SIZE: 166 amino acids; 19348 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
PTM: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
DISEASE: SwissProt: P01579 # In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
SIMILARITY: SwissProt: P01579 ## Belongs to the type II (or gamma) interferon family.
Stem Cell Type
  • Mesenchymal Stem Cells
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IF002 04053252284038

Documentation

Interferon-γ Protein, Recombinant human SDS

Title

Safety Data Sheet (SDS) 

Interferon-γ Protein, Recombinant human Certificates of Analysis

TitleLot Number
RECOMBINANT HUMAN INTERFERON-#947; - 2120010 2120010
RECOMBINANT HUMAN INTERFERON-g - 2045844 2045844
RECOMBINANT HUMAN INTERFERON-g - 2063151 2063151
RECOMBINANT HUMAN INTERFERON-g - 2196000 2196000
RECOMBINANT HUMAN INTERFERON-g - 2295640 2295640
RECOMBINANT HUMAN INTERFERON-g - 2309018 2309018
RECOMBINANT HUMAN INTERFERON-y 2915313
RECOMBINANT HUMAN INTERFERON-y 2942616
RECOMBINANT HUMAN INTERFERON-y 2886670
RECOMBINANT HUMAN INTERFERON-γ 3069347
Show more

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors