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IPSN07852 Immobilon®-P Blotting Sandwich

7 x 8.4 cm, Immobilon-P membrane interleaved with blotting paper, for standard tank transfer procedures

Immobilon®-P Blotting Sandwich MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
IPSN07852
20  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
WB, DB
Description
Catalogue NumberIPSN07852
Trade Name
  • Immobilon
DescriptionImmobilon®-P Blotting Sandwich
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information
Detection methodChemiluminescence Chromogenic Radioactive Colorimetric
Compatible Stains
  • Transillumination
  • Toluidine blue
  • Sypro® ruby
  • Ponceau-S red
  • India ink
  • Coomassie brilliant blue
  • Colloidal gold
Filter CodeIPSN
Filter ColorWhite
Quality LevelMQ400
Applications
Application7 x 8.4 cm, Immobilon-P membrane interleaved with blotting paper, for standard tank transfer procedures
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
BSA Adsorption215 µg/cm²
Goat IgG Adsorption294 µg/cm²
Insulin Adsorption160 µg/cm²
WettabilityHydrophobic
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions7 cm x 8.4 cm
Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
  • Paper
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size20
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IPSN07852 04053252369919