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401002 Iberiotoxin, Buthus tamulus, Recombinant, E. coli - CAS 129203-60-7 - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ

Primary Target: High conductance Ca2+-activated K+ channels
401002
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Overview

Replacement Information

Key Spec Table

CAS #
129203-60-7

Products

Catalogue NumberPackaging Qty/Pack
401002-10UG Plastic ampoule 10 μg
Description
OverviewRecombinant Buthus tamulus iberiotoxin expressed in E. coli. Acts as a selective, reversible blocker of high conductance Ca2+-activated K+ channels (IC50 = 250 pM).
Catalogue Number401002
Brand Family Calbiochem®
SynonymsrlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
References
ReferencesVanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.
Product Information
CAS number129203-60-7
ATP CompetitiveN
FormSalt-free lyophilized solid
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Biological activitypotently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes
Primary TargetHigh conductance Ca2+-activated K+ channels
Primary Target IC<sub>50</sub>250 pM against high conductance Ca2+-activated K+ channels
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequencePyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
401002-10UG 04055977189360

Documentation

Iberiotoxin, Buthus tamulus, Recombinant, E. coli - CAS 129203-60-7 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Iberiotoxin, Buthus tamulus, Recombinant, E. coli - CAS 129203-60-7 - Calbiochem Certificates of Analysis

TitleLot Number
401002

References

Reference overview
Vanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-July-2008 RFH
SynonymsrlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
DescriptionRecombinant Buthus tamulus iberiotoxin expressed in E. coli. A single polypeptide chain of 37 amino acids that displays about 68% sequence homology with charybdotoxin (ChTx). Acts as a selective, reversible blocker of high conductance, Ca2+-activated K+ channels (PK,Ca). rIbTx does not block other types of voltage-dependent ion channels, especially other types of K+ channels that are sensitive to inhibition by ChTx. Shown to block PK,Ca in excised membrane patches from bovine aortic smooth muscle, acting exclusively at the outer face of the channel (IC50 = 250 pM). Causes a decrease in probability of channel opening and the channel open mean time. rIbTx appears to modulate ChTx binding by an allosteric mechanism.
FormSalt-free lyophilized solid
CAS number129203-60-7
Peptide SequencePyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)
Purity≥98% by HPLC
Biological activitypotently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes
SolubilityReconstitute in Tris-buffered saline (100 mM NaCl, 10 mM Tris-HCl, pH 7.5) containing 0.1% BSA.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Toxicity Harmful
ReferencesVanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.