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EZHPI-15BK Human Total Proinsulin ELISA- Space Saver (Bulk) Packaging

This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.

EZHPI-15BK
Ten 96-well strip plates.  This product contains 10 Total Proinsulin ELISA kits.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberEZHPI-15BK
DescriptionHuman Total Proinsulin ELISA- Space Saver (Bulk) Packaging
Background InformationProinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.
References
Product Information
Detection methodFluorescent Colorimetric
Precision, %
  • Inter-assay: 2.9–8.3
  • Intra-assay: 0.8–8.5
Quality LevelMQ200
Applications
ApplicationThis Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.
Key Applications
  • ELISA
Application Notes20 μL sample volume 3 hour assay, room temperature
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Proinsulin
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 88–103%
Physicochemical Information
Sensitivity
  • 0.5 pM
Linearity of Dilution84–120%
Standard Curve Range
  • 2–200 pM
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material SizeTen 96-well strip plates.
Material PackageThis product contains 10 Total Proinsulin ELISA kits.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZHPI-15BK 04053252436994

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