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SCR108 Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow

This Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCR108
1 kit containg >1 million viable cells and 500 mL of optimized media  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR108
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHuman Mesenchymal Stem Cell Kit - Derived from Bone-Marrow
References
Product Information
Quality LevelMQ100
Applications
ApplicationThis Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media.
Key Applications
  • Stem Cell Culture
Application NotesMesenchymal stem cells, also known as marrow stromal cells (1), are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues (1, 2). Mesenchymal stem cells also provide support and maintenance for the other major stem cell population in the bone marrow, the hematopoietic stem cells (2).
Mesenchymal stem cells (MSC’s) have historically been isolated based on the ability of these cells to form adherent cell layers in culture and the concomitant lack of adherence of other cells in the bone marrow stroma such as hematopoietic stem cells, adipocytes, and macrophage (1, 3). While this procedure results in enriched populations of mesenchymal stem cells, the resulting bone-marrow derived cell populations are, nonetheless, heterogeneous – comprised not only of mesenchymal stem cells, but also of committed lineage-restricted progenitors (1, 3). To aid researchers in the accurate identification and characterization of mesenchymal stem cells, Millipore provides cryopreserved Human Mesenchymal Stem Cells and optimized media in this kit. Millipore’s cryopreserved Human Mesenchymal Stem Cells are ready-to-use primary mesenchymal stem cells isolated from the iliac crest of normal human bone marrow (18-30 years old).
Biological Information
SourceHuman bone marrow
Stem Cell Type
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceMillipore’s Human Mesenchymal Stem Cells are derived from human bone marrow and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), THY-1 (CD90) and STRO-1 and for their absence of hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes). Millipore’s Human Mesenchymal Stem Cells are also negative for the endothelial marker, M-CAM (CD146). The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma. Each lot of Mesenchymal Stem Cell medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsHuman Mesenchymal Stem Cells (Part # SCC034) should be stored in liquid nitrogen.
Mesenchymal Stem Cell Expansion Medium: (Catalog No. SCM015) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
Packaging Information
Material Size1 kit containg >1 million viable cells and 500 mL of optimized media
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR108 04053252005190

Documentation

Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Self-organizing tissue-engineered constructs in collagen hydrogels.
Robert G Gourdie,Tereance A Myers,Alex McFadden,Yin-Xiong Li,Jay D Potts
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada  18  2012

Show Abstract 		22214557 22214557