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EZGRA-88BK Human Ghrelin (active) ELISA- Bulk

This Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.

Human Ghrelin (active) ELISA- Bulk MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
EZGRA-88BK
Ten 96-well strip plates  This product consists of ten Ghrelin (active) ELISA kits.
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Ghrelin (Active) HELISAFluorescent Colorimetric
Description
Catalogue NumberEZGRA-88BK
DescriptionHuman Ghrelin (active) ELISA- Bulk
Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
References
Product Information
Detection methodFluorescent Colorimetric
Precision, %
  • Inter-assay: Serum: 3.5–6.6% Plasma: 7.5–12.9%
  • Intra-assay: Serum: 1.6–3.6% Plasma: 0.9–7.5%
Quality LevelMQ200
Applications
ApplicationThis Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • ELISA
Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Biological Information
SpecificityHuman Ghrelin (active) 100% Des-Octanoyl Human Ghrelin not detectable Canine Ghrelin (active) 106% Porcine Ghrelin (active) 57% Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
Species Reactivity
  • Human
Analytes Available
  • Ghrelin (Active)
Entrez Gene Number
Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
Gene Symbol
  • GHRL
  • ghrelin
  • obestatin
  • MTLRP
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Accuracy
  • Serum: 84.4–88.4%
  • Plasma: 94.7–94.8%
Physicochemical Information
Sensitivity
  • 8 pg/mL (20 µL sample size)
Linearity of DilutionSerum: 100.1–110.2% Plasma: 87.5–89.9%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material SizeTen 96-well strip plates
Material PackageThis product consists of ten Ghrelin (active) ELISA kits.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZGRA-88BK 04053252436895