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GHRT-89HK Human Ghrelin (TOTAL) RIA

GHRT-89HK
125 tubes  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Ghrelin HRIARadioactive 125I
Description
Catalogue NumberGHRT-89HK
DescriptionHuman Ghrelin (TOTAL) RIA
Background InformationGhrelin is a recently discovered hormone is secreted primarily by the stomach in response to glucose administration or CNS signals.
Product Information
Detection methodRadioactive 125I
Performance
  • ED80 = 395 ± 88 pg/mL
  • ED50 = 895 ± 177 pg/mL
  • ED20 = 2015 ± 382 pg/mL
Precision, %
  • Inter-assay: 14.7–17.8
  • Intra-assay: 3.3–10.0
Quality LevelMQ100
Applications
ApplicationThis radioimmunoassay (RIA) measures & quantifies Ghrelin for use in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • Radioimmunoassay
Application NotesTwo-day disequilibrium assay at 4°C. Sample volume: < 100 μL Plasma (collected on ice) recommended
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Ghrelin
Entrez Gene Number
Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
Gene Symbol
  • GHRL
  • ghrelin
  • MTLRP
  • obestatin
Radioactive Isotype125I-Human Ghrelin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Accuracy
  • 90–96%
Physicochemical Information
Sensitivity
  • 100–10,000 pg/mL
Linearity of Dilution99–146%
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size125 tubes
Global Trade Item Number
Catalogue Number GTIN
GHRT-89HK 04053252404047