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SCC265 Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line

Huh-7.5 Tet-On human hepatocellular carcinoma cell line is useful for Hepatitis C virus (HCV) studies and production.

SCC265
≥1X10⁶ cells/vial   
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCC265
DescriptionHuh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line
Alternate Names
  • Huh7.5
  • Huh 7.5
Background InformationHuh-7 is a well differentiated hepatocyte-derived carcinoma cell line, isolated from the liver tumor of a 57-year-old Japanese male (Nakabayashi, 1982). Derived from Huh-7, the Huh-7.5 cell line is highly permissive for subgenomic and genomic Hepatitis C virus (HCV) RNA replication (Blight et al., 2002) and is now employed for high yield production of HCV and studies of antivirals on industrial scale.

Huh-7.5 Tet-On Human Hepatocellular Carcinoma Cell Line was generated from Huh-7.5 cells using retroviral transduction to express the Tet-On 3G transactivator protein and enable subsequent doxycycline-regulated transgene expression to aid in the study of subcellular interactions between HCV and its host (Luna, 2015; Takacs, 2017).
References
Product Information
Applications
ApplicationHuh-7.5 Tet-On human hepatocellular carcinoma cell line is useful for Hepatitis C virus (HCV) studies and production.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC265 04061841827979