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12-347 Histone H4 Peptide

Histone H4 Peptide primarily used in Histone Acetyltransferase Assays (HAT) and Western Blotting.

Histone H4 Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Histone Acetyltransferase Assay (HAT), Western Blotting UniProt Number: P62805 Entrez Gene Number: NM_003538.3
12-347
100 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsEntrez Gene NumberUni Prot Number
HAT, WBNM_003538.3 P62805
Description
Catalogue Number12-347
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHistone H4 Peptide
References
Product Information
PresentationLyophilized powder
Quality LevelMQ100
Applications
ApplicationHistone H4 Peptide primarily used in Histone Acetyltransferase Assays (HAT) and Western Blotting.
Key Applications
  • Histone Acetyltransferase Assay (HAT)
  • Western Blotting
Biological Information
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Immunoblot Analysis. This peptide can be used as a standard for antibodies specific for the N-terminus of histone H4. It can also be chemically acetylated with [3H]CH3COONa using the HDAC Assay Kit (17-281). The radiolabeled peptide was used to assay HDAC activity in a HeLa nuclear extract (12-309).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-347 04053252334504

Documentation

Histone H4 Peptide SDS

Title

Safety Data Sheet (SDS) 

Histone H4 Peptide Certificates of Analysis

TitleLot Number
Histone H4 Peptide 2487892
Histone H4 Peptide (substrate for HAT activity) - 2459574 2459574
Histone H4 Peptide (substrate for HAT activity) 2964463
Histone H4 Peptide - 16515 16515
Histone H4 Peptide - 18894 18894
Histone H4 Peptide - 20638 20638
Histone H4 Peptide - 21828 21828
Histone H4 Peptide - 3319768 3319768
Histone H4 Peptide - 3944858 3944858
Histone H4 Peptide - 4179410 4179410
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References

Reference overviewPub Med ID
The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.
Mizzen, C A, et al.
Cell, 87: 1261-70 (1996)  1996

Show Abstract 		8980232 8980232

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Alternative Format

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Categories

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Control Histones