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03-185 Histone H2B human recombinant

Human recombinant H2B expressed in E. Coli.

Histone H2B human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Kinase Assay, DNA-binding Proteins, Western Blotting
03-185
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
KA, DNA-binding Proteins, WB
Description
Catalogue Number03-185
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHistone H2B human recombinant
OverviewHuman recombinant H2B expressed in E. Coli
Background InformationHistone H2B is one of four core histones that form an octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability and consequently, transcription, and DNA replication and repair. The most widely studied of these modifications are methylation, phosphorylation, and acetylation which may produce different effects on gene regulation depending on the number and pattern of modifications. Histone H2B undergoes acetylation at lysines 5, 15, and 20. It is also phosphorylated in apoptotic cells during DNA fragmentation, possibly in a caspase-dependent manner.
References
Product Information
FormatPurified
PresentationLiquid in Dulbecco's PBS. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant H2B expressed in E. Coli.
Key Applications
  • Kinase Assay
  • DNA-binding Proteins
  • Western Blotting
Application NotesChromatin assembly, Western blot (control), Kinase Assay
Biological Information
HostE. coli
PurityPurity >95% by SDS-PAGE
Molecular Weight~17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-185 04053252550799