Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF116 Hepatocyte Growth Factor Protein, Recombinant human

The Human Hepatocyte Growth Factor (or HGF protein) is also known as Scatter Factor & Hepatopoeitin A.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P14210 Entrez Gene Number: NM_000601.4, NM_001010931.1, NM_001010932.1, NM_001010933.1, NM_001010934.1
GF116
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman Baculovirus infected High-5 cellsGreater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Description
Catalogue NumberGF116
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHepatocyte Growth Factor Protein, Recombinant human
OverviewHuman Hepatocyte Growth Factor (HGF) is also known as Scatter Factor and Hepatopoeitin A. HGF is a potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive.

GF116 is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (234 amino acids).
Alternate Names
  • HGF
References
Product Information
HS Code3504 00 90
PresentationThe sterile filtered solution was lyophilized from 0.1M L-arginine HCl, 10 mM Tris, pH 8.0, and 200 mM NaCl.
Quality LevelMQ100
Applications
ApplicationThe Human Hepatocyte Growth Factor (or HGF protein) is also known as Scatter Factor & Hepatopoeitin A.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
SourceBaculovirus infected High-5 cells
Specific ActivityThe ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.
Entrez Gene Number
Entrez Gene SummaryHepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
Gene Symbol
  • HGF
  • F-TCF
  • Hepatopoeitin-A
  • HGFB
  • HPTA
  • SF
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P14210 # HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.
SIZE: 728 amino acids; 83134 Da
SUBUNIT: Dimer of an alpha chain and a beta chain linked by a disulfide bond.
SIMILARITY: SwissProt: P14210 ## Belongs to the peptidase S1 family. Plasminogen subfamily. & Contains 4 kringle domains. & Contains 1 PAN domain. & Contains 1 peptidase S1 domain.
Stem Cell Type
  • Mesenchymal Stem Cells
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted HGF should be stored in working aliquots at -20°C. We recommend a quick centrifugation of the vial followed by reconstitution in water to a concentration of 0.5 mg/mL. This solution can then be diluted into other buffers and stored at 4°C for 1 week or stored at -20°C for future use. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF116 08436037125737

Documentation

Hepatocyte Growth Factor Protein, Recombinant human SDS

Title

Safety Data Sheet (SDS) 

Hepatocyte Growth Factor Protein, Recombinant human Certificates of Analysis

TitleLot Number
GF116 RECOMBINANT HUMAN HEPATOCYTE -2739424 2739424
Hepatocyte Growth Factor Protein, Recombinant human - 3898591 3898591
Hepatocyte Growth Factor Protein, Recombinant human - 3916178 3916178
Hepatocyte Growth Factor Protein, Recombinant human - 3934926 3934926
Hepatocyte Growth Factor Protein, Recombinant human - 3955277 3955277
Hepatocyte Growth Factor Protein, Recombinant human - 4004371 4004371
Hepatocyte Growth Factor Protein, Recombinant human - 4009729 4009729
Hepatocyte Growth Factor Protein, Recombinant human - 4021704 4021704
Hepatocyte Growth Factor Protein, Recombinant human - 4029960 4029960
Hepatocyte Growth Factor Protein, Recombinant human - 4034277 4034277
Show more

Data Sheet

Title
RECOMBINANT HUMAN HEPATOCYTE GROWTH FACTOR

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors