Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

F0483-BC HAT-Supplement

F0483-BC
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
OverviewHAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway.
Specifications:

Sterility: Pass
pH: 9.0-11
Osmolality: 0.265-0.295 Osm/kg H20
Color: Colorless
Appearance: Clear
Catalogue NumberF0483-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
F0483-BC 0

Documentation