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109218 Gram's crystal violet solution

for the Gram staining method

Gram's crystal violet solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109218
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092180500 Plastic bottle 500 ml
1092182500 Plastic bottle 2.5 l
Description
Catalogue Number109218
DescriptionGram's crystal violet solution
OverviewGram's crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram's crystal violet solution (Cat. No.109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat No.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. No. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram's safranine solution (Cat.no. 109217) and will then appear pink to red.
A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the Gram staining method
Biological Information
Physicochemical Information
Density0.99 g/cm3 (20 °C)
Flash point47 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H226: Flammable liquid and vapour.
H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
P242: Use non-sparking tools.
P273: Avoid release to the environment.
Signal WordWarning
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal28
Aqueous solutions: Container D.
Safety Information
Categories of dangerflammable, dangerous for the environment
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDKein Gefahrgut
Declaration (transport by air) IATA-DGRNo Dangerous Good
Declaration (transport by sea) IMDG-CodeNo Dangerous Good
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red
Global Trade Item Number
Catalogue Number GTIN
1092180500 04022536112077
1092182500 04022536557403

Related Products & Applications

Alternative Products

Catalogue Number Description
111885 Gram-Color

Related Products

Catalogue Number Description
111885 Gram-Color

Accessories (Primary)

Catalogue Number Description
100567 Lugol's solution
109217 Gram's safranine solution
109261 Lugol's solution (diluted iodine-potassium iodide solution)
110218 Gram's decolorizing solution

Accessories (Secondary)

Catalogue Number Description
100567 Lugol's solution
109217 Gram's safranine solution
109261 Lugol's solution (diluted iodine-potassium iodide solution)
111885 Gram-Color

Related Products By: Application Facete

Gram bacteria Detection
Bacteria Staining

Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining