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354104 Glutathione Peroxidase, Cellular Assay Kit

Glutathione Peroxidase, Cellular Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Description
Overview

This product has been discontinued.

We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.





A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH.
Each kit can be used to perform up to 100 assays.
Catalogue Number354104
Brand Family Calbiochem®
Application Data
Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C.
Materials Required but Not Delivered• Spectrophotometer, preferably equipped with a temperature-controlled cuvette chamber capable of measuring the absorbance at 340 nm. • Cuvettes with 1-cm path length
References
ReferencesThompson, C.D., et al. 1977. Br. J. Nutr. 37, 457. Paglia, D.E., and Valentine, W.N. 1967. J. Lab. Clin. Med. 70, 158.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm.
Detection methodColorimetric
Form100 Tests
FormatCuvette
Kit containsGlutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol.
Biological Information
Assay range5.6-24 mU/ml
Assay time2 h
Sample TypeCell lysates, tissue extracts, erythrocyte lysates
Safety Information
R PhraseR: 8-20/21/22-34-40-44

Contact with combustible material may cause fire.
Harmful by inhalation, in contact with skin and if swallowed.
Causes burns.
Limited evidence of a carcinogenic effect.
Risk of explosion if heated under confinement.
S PhraseS: 23-36/37/39-16-17

Do not breathe fumes.
Wear suitable protective clothing, gloves and eye/face protection.
Keep away from sources of ignition - No Smoking.
Keep away from combustible material.
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple Storage Requirements
Do not freeze Ok to freeze
Supplemental Information
Kit containsGlutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
354104 0