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GF003AF-MG Sigma-AldrichFibroblast Growth Factor basic Protein, Human recombinant (mg qty.)

The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

UniProt Number: P09038 Entrez Gene Number: NM_002006.4

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Overview

Replacement Information

Key Spec Table

Species	Source	Purity
Human	expressed in E. coli	Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF.

Description
Catalogue Number	GF003AF-MG
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.)
Alternate Names	FGF-2 bFGF basic-FGF Heparin-Binding Growth Factor 2 Prostatropin
Background Information	Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.

References

Product Information
HS Code	2937 19 90
Presentation	Lyophilized from a solution of 5mM Tris, pH 7.6 with 150 mM NaCl.
Quality Level	MQ100

Applications
Application	The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
Application Notes	For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/ml, depending on the method of culture

Biological Information
Purity	Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of bFGF.
Source	expressed in E. coli
Specific Activity	Chemicon's Human FGF-b is fully biologically active when compared to standards. The ED<sub>50</sub> was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors.
Entrez Gene Number	NM_002006.4
Entrez Gene Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Gene Symbol	FGF2 prostatropin Prostatropin HBGF-2 BFGF FGFB
UniProt Number	P09038
UniProt Summary	FUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SIZE: 155 amino acids; 17254 Da SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
Stem Cell Type	Neural Stem Cells Epithelial Cells Mesenchymal Stem Cells Pancreatic Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain the lyophilized material at -20°C until expiration date as stated on the label. General applications: After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity. For Human ES cell culture: After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).

Storage and Shipping Information

Storage Conditions

Maintain the lyophilized material at -20°C until expiration date as stated on the label.

General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20°C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.

For Human ES cell culture:
After a quick spin, reconstitute to 10 μg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture).

Packaging Information
Material Size	1 mg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
GF003AF-MG	04053252326578

Documentation

Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.) SDS

Title
Safety Data Sheet (SDS)

Fibroblast Growth Factor basic Protein, Human recombinant (mg qty.) Certificates of Analysis

Title	Lot Number
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2330574	2330574
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2390531	2390531
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 2455620	2455620
RECOMBINANT ANIMAL-FREE -2508896	2508896
RECOMBINANT ANIMAL-FREE -2700962	2700962
RECOMBINANT ANIMAL-FREE -2746009	2746009
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC	2934834
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC	2474982
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC	2846668
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC - 1992395	1992395

References

Reference overview	Pub Med ID
Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells Xu, Ren-He, et al Nat Methods, 2:185-190 (2005) 2005	15782187

Data Sheet

Title
RECOMBINANT ANIMAL-FREE HUMAN FIBROBLAST GROWTH FACTOR-BASIC

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