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S-014-B Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml

The Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S-014-B
500 mL  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberS-014-B
Trade Name
  • Specialty Media
DescriptionEnzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml
OverviewEnzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents and agents to stabilize their activity on cells. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.



Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
Product Information
PresentationProduct is supplied as a sterile

liquid.
Quality LevelMQ200
Applications
ApplicationThe Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsProduct can be stored at room temperature or at 4°C. Shelf life is 2 years
Packaging Information
Material Size500 mL
Global Trade Item Number
Catalogue Number GTIN
S-014-B 04053252579783