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SF016-200 ESGRO-2i Medium, 200ml

ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.

ESGRO-2i Medium, 200ml MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SF016-200
200 mL  
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Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSF016-200
DescriptionESGRO-2i Medium, 200ml
OverviewTwo stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs.
ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for cell expansion and growth at clonal density in serum-free conditions and supports a naive/ground pluripotent state.
Alternate Names
  • 2i Naive ES/IPS Media
References
Product Information
Components
  • 1. ESGRO-2i Basal Medium with LIF: (Catalogue No. CS204463) Two (2) 100 mL bottles containing basal medium supplemented with LIF, pH 7.1-7.3.
  • 2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) Two (2) 50 μL vials containing selective GSK3β inhibitor supplement.
  • 3. Mek1/2 Inhibitor Supplement: (Catalogue No. CS204465) Two (2) 10 μL vials containing selective Mek 1/2 inhibitor supplement.
HS Code3821 00 00
Quality LevelMQ100
Applications
ApplicationESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. Supports a naive/ground pluripotent state.
Key Applications
  • Stem Cell Culture
Application NotesSuitable for ES and iPS cell derivation, passaging and maintenance. Media can be used on feeder dependent and independent pluirpotent stem cell lines.

Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Biological Information
Media FormLiquid
Stem Cell Type
  • Induced Pluripotent Stem Cells
  • Mouse Embryonic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions• Upon receipt, store basal medium and inhibitor supplements in the dark at -20°C until use. Refer to lot expiration date on labels.
• After basal medium and inhibitor supplements are combined, store the complete ESGRO-2i medium in the dark at 4°C for up to 10 days. Do not refreeze.
Packaging Information
Material Size200 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SF016-200 04053252021015