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2116 Detergent-OUT™ Med Kit

Detergent-OUT™ Med Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Clean-up
2116
1 kit  10 columns
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
PCU
Description
Catalogue Number2116
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionDetergent-OUT™ Med Kit
OverviewThe Chemicon Detergent-OUT Kit is a simple high performance method for removing detergents from protein solutions. Removes detergents without significant dilution of protein solution. Simply load the protein solution on the Detergent-OUT Spin column and collect detergent free protein solution. The Med column is suitable for removing detergents from a 1.0 mL solution of 1.5% Triton-X100 detergents (or an equivalent of 15 μL Triton-X100 or 15 mg detergents), provided that both the columns and proteins solutions are prepared in water.
Materials Required but Not DeliveredCentrifuge

Centrifuge tubes

Collection tubes
References
Product Information
Components
  • Detergent-OUT Columns-DTG-100X 10 Med
Applications
Key Applications
  • Protein Clean-up
Application NotesPROTOCOL:

1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.

2. Buffer Equilibration: Detergent-OUT columns are supplied in deionized water. A better result is obtained when the column and protein solution are in water or phosphate buffer. Columns may be equilibrated with other types of buffer. Note: Do not equilibrate the columns with Tris buffer and other such buffers. Apply about 1.0 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.

3. Place the column in a 15mL centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.

4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 5 minutes..

5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging Information
Material Size1 kit
Material Package10 columns
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
2116 04053252276163

Documentation

Detergent-OUT™ Med Kit Certificates of Analysis

TitleLot Number
DETERGENT-OUT KIT (MED) - 2561136 2561136
DETERGENT-OUT KIT (MED) - 3321992 3321992
DETERGENT-OUT KIT (MED) - 3330378 3330378
DETERGENT-OUT KIT (MED) - 3383279 3383279
DETERGENT-OUT KIT (MED) - 3429556 3429556
DETERGENT-OUT KIT (MED) - 3444443 3444443
DETERGENT-OUT KIT (MED) - 3506772 3506772
DETERGENT-OUT KIT (MED) - 3539502 3539502
DETERGENT-OUT KIT (MED) - 3605546 3605546
DETERGENT-OUT KIT (MED) - 3610044 3610044
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Data Sheet

Title
DETERGENT-OUT KIT (MED)