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260905 DNase γ Inhibitor, 6-DTAF

DNase γ Inhibitor, 6-DTAF MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4-(4,6-Dichloro-[1,3,5]-triazin-2-ylamino)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid, 6-(4,6-Dichlorotriazinyl)aminofluorescein, DR396

260905
  
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Overview

Replacement Information
Description
Overview

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A cell-permeable triazinyl-aminofluorescein compound that acts as a potent and selective inhibitor of DNase γ, human (IC50 = 3.2 µM) with minimal effects on DNase I, bovine (Cat. No. 260913; IC50 > 300 µM). Shown to prevent DNase γ-catalyzed apoptotic DNA fragmentation in HeLa-γ cells induced by Staurosporine (Cat. No. 569397) and in rat splenocytes exposed to γ-ray irradiation. Does neither affect PARP cleavage nor CAD (caspase activated DNase)-mediated DNA fragmentation.
Catalogue Number260905
Brand Family Calbiochem®
Synonyms4-(4,6-Dichloro-[1,3,5]-triazin-2-ylamino)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid, 6-(4,6-Dichlorotriazinyl)aminofluorescein, DR396
References
ReferencesOkamoto, M., et al. 2005. Biochem. Biophys. Res. Commun. 327, 76. Sunaga, S., et al. 2004. Biochem. Biophys. Res. Commun. 325, 1292.
Product Information
CAS number82169-58-2
FormYellow solid
Hill FormulaC₂₃H₁₂Cl₂N₄O₅
Chemical formulaC₂₃H₁₂Cl₂N₄O₅
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetDnase γ, human
Primary Target IC<sub>50</sub>3.2 µM
Secondary targetDnase-1,bovine = 300 µM
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
260905 0

Documentation

DNase γ Inhibitor, 6-DTAF Certificates of Analysis

TitleLot Number
260905

References

Reference overview
Okamoto, M., et al. 2005. Biochem. Biophys. Res. Commun. 327, 76. Sunaga, S., et al. 2004. Biochem. Biophys. Res. Commun. 325, 1292.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2008 RFH
Synonyms4-(4,6-Dichloro-[1,3,5]-triazin-2-ylamino)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoic acid, 6-(4,6-Dichlorotriazinyl)aminofluorescein, DR396
DescriptionA cell-permeable, potent, and selective inhibitor of human DNase γ (IC50 = 3.2 µM) with minimal effects on DNase I, Bovine (Cat. No. 260913; IC50 >300 µM). Shown to prevent DNase γ-catalyzed apoptotic DNA fragmentation in rat splenocytes exposed to γ-ray irradiation and in HeLa cells transfected with DNAse γ and induced by Staurosporine (Cat. No. 569397). Does not affect PARP cleavage or CAD (caspase activated DNase)-mediated DNA fragmentation.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number82169-58-2
Chemical formulaC₂₃H₁₂Cl₂N₄O₅
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (15 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Carcinogenic / Teratogenic
ReferencesOkamoto, M., et al. 2005. Biochem. Biophys. Res. Commun. 327, 76. Sunaga, S., et al. 2004. Biochem. Biophys. Res. Commun. 325, 1292.