Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

268298 DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem

Cell-permeable DNA-binding dye.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4ʹ,6-Diamidino-2-phenylindole, 2HCl

268298
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
28718-90-3C₁₆H₁₅N₅ · 2HCl

Products

Catalogue NumberPackaging Qty/Pack
268298-10MG Plastic ampoule 10 mg
Description
OverviewCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Catalogue Number268298
Brand Family Calbiochem®
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
References
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.
Product Information
CAS number28718-90-3
ATP CompetitiveN
FormYellow solid
Hill FormulaC₁₆H₁₅N₅ · 2HCl
Chemical formulaC₁₆H₁₅N₅ · 2HCl
Hygroscopic Hygroscopic
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetBinds preferentially to DNA rich in adenine and thymine
Purity≥90% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNL5995050
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
268298-10MG 04055977197785

Documentation

DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem Certificates of Analysis

TitleLot Number
268298

References

Reference overview
Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-June-2008 RFH
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl
DescriptionFluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
FormYellow solid
CAS number28718-90-3
RTECSNL5995050
Chemical formulaC₁₆H₁₅N₅ · 2HCl
Structure formulaStructure formula
Purity≥90% by HPLC
SolubilityEthanol, H₂O (5 mg/ml), or Methanol
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.