Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

S8005 CpGenome™ 5-mC and 5-hmC DNA Standard Set

This set can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.

CpGenome™ 5-mC and 5-hmC DNA Standard Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S8005
2 µg per vial  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberS8005
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ 5-mC and 5-hmC DNA Standard Set
OverviewCpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.

Materials Provided:

Three vials, each containing 2 µg (40 µL) of CpGenome™ Cytosine DNA Standard, CpGenome™ 5-mC DNA Standard, or CpGenome™ 5-hmC DNA Standard, at a concentration of 50 ng/μL.

Validation:

The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.
Product Information
PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThis set can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.
Biological Information
Species Reactivity
  • Human
Modifications
  • Methylation
  • Hydroxylation
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended Storage: Stable for 6 months at -20°C from date of receipt.
Packaging Information
Material Size2 µg per vial
Global Trade Item Number
Catalogue Number GTIN
S8005 04053252648427