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HTS089M ChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

Human LPA1 / EDG2 GPCR membrane preparation for GTPγS binding Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: Q92633 Entrez Gene Number: NM_001401
HTS089M
200 units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1LPA1 / EDG2
Description
Catalogue NumberHTS089M
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiSCREEN™ Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor
OverviewHuman EDG2 encoding LPA1
Background InformationLysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA1 is widely expressed, with particularly notable expression in the ventricular zone of the embryonic cerebral cortex. Mice lacking LPA1 exhibit craniofacial abnormalities and defective development of the cerebral cortex (Harrison et al., 2003; Choi et al., 2008). LPA1 also promotes fibrotic responses to tissue injury (Tager et al., 2008)

Millipore's LPA1 Membrane Preparation is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s LPA1 Calcium Optimized Cell Line (HTS089C). This LPA1 Membrane Preparation exhibits both constitutive and agonist-induced activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist, inverse agonist, and antagonist activities. The membrane preparations exhibits EC50 of 73 nM for LPA in a GTPγS binding assay.
References
Product Information
FormatMembranes
PresentationOne package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific LPA-stimulated [35S]-GTPγS binding.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman LPA1 / EDG2 GPCR membrane preparation for GTPγS binding Assays.
Key Applications
  • GTPγS binding
Application NotesGTPγS binding assay
Biological Information
Entrez Gene Number
Gene Symbol
  • LPAR1
  • EDG2
  • LPA1
GPCR ClassA
Host CellsChem-1
Protein TargetLPA1 / EDG2
Target Sub-FamilyLysophospholipid
UniProt Number
Incubation ConditionsGTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/10 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance1 unit = 5 μg
EC50 in GTPγS binding assay by LPA: ~ 73 nM
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS089M 04053252367649

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