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627610 Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem

Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-VAD (OMe)-FMK

Primary Target: Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
627610
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
187389-52-2C₂₂H₃₀FN₃O₇

Products

Catalogue NumberPackaging Qty/Pack
627610-1MG Plastic ampoule 1 mg
627610-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable, irreversible, pan-caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells. Shown to enhance the freeze-thaw survival of human embryonic stem cells. When using with purified native or recombinant enzyme, pre-treatment with an esterase is required.
Catalogue Number627610
Brand Family Calbiochem®
SynonymsZ-VAD (OMe)-FMK
References
ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
Martin, U., et al. 2007. Apoptosis 12, 525.
Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
Cain, K., et al. 1996. Biochem J. 314, 27.
Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
Pronk, G.J., et al. 1996. Science 271, 808.
Slee, E.A., et al. 1996. Biochem. J. 315, 21.
Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
Zhu, H., et al. 1995. FEBS Lett. 374, 303.
Product Information
CAS number187389-52-2
ATP CompetitiveN
FormWhite solid
Hill FormulaC₂₂H₃₀FN₃O₇
Chemical formulaC₂₂H₃₀FN₃O₇
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetFas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells
PuritySingle spot by TLC
Physicochemical Information
Cell permeableY
Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
627610-1MG 04055977262810
627610-5MG 04055977262841

Documentation

Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem Certificates of Analysis

TitleLot Number
627610

References

Reference overview
Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
Martin, U., et al. 2007. Apoptosis 12, 525.
Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
Cain, K., et al. 1996. Biochem J. 314, 27.
Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
Pronk, G.J., et al. 1996. Science 271, 808.
Slee, E.A., et al. 1996. Biochem. J. 315, 21.
Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
Zhu, H., et al. 1995. FEBS Lett. 374, 303.

Citations

Title
  • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
  • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-February-2012 RFH
    SynonymsZ-VAD (OMe)-FMK
    DescriptionA cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
    FormWhite solid
    CAS number187389-52-2
    Chemical formulaC₂₂H₃₀FN₃O₇
    Peptide SequenceZ-Val-Ala-Asp(OMe)-CH₂F*
    Structure formulaStructure formula
    PuritySingle spot by TLC
    SolubilityDMSO (50 mM)
    Storage Protect from moisture
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
    Toxicity Standard Handling
    ReferencesHeng, B.C., et al. 2007. Biosci. Rep. 27, 257.
    Martin, U., et al. 2007. Apoptosis 12, 525.
    Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.
    Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
    McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
    Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
    Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
    An, S. and Know, K.A. 1996. FEBS Lett. 386, 115.
    Cain, K., et al. 1996. Biochem J. 314, 27.
    Mirzoeva, O.K., et al. 1996. FEBS Lett. 396, 266.
    Pronk, G.J., et al. 1996. Science 271, 808.
    Slee, E.A., et al. 1996. Biochem. J. 315, 21.
    Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
    Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
    Zhu, H., et al. 1995. FEBS Lett. 374, 303.
    Citation
  • Heng, B.C., et al. 2007. Biosci. Rep. 27, 257.
  • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
    		•