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218720 Caspase-4, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ICErel-II, ICH-2, TX

218720
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human caspase-4 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 19000.
M.W. 10,000 and 19,000 (heterodimer).
Catalogue Number218720
Brand Family Calbiochem®
SynonymsICErel-II, ICH-2, TX
References
ReferencesKamada, S., et al. 1997. Oncogene, 15, 285.
Nasir, J., et al. 1997. Mamm. Genome 8, 611.
Nath, R., et al. 1996. Biochem. J. 319, 683.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-<i>p</i>NA (Cat. No. 218805) per min at 25°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218720 0

Documentation

Caspase-4, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218720

References

Reference overview
Kamada, S., et al. 1997. Oncogene, 15, 285.
Nasir, J., et al. 1997. Mamm. Genome 8, 611.
Nath, R., et al. 1996. Biochem. J. 319, 683.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-January-2008 JSW
SynonymsICErel-II, ICH-2, TX
DescriptionRecombinant, human caspase-4 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. Member of the caspase-1 or ICE (interleukin-1β-converting enzyme) subfamily that is involved in Fas-mediated apoptosis. Cleavage sites of the maturation process of caspase-4 are located at Asp104, Asp270, and Asp289. Interacts with Apaf-1 and participates in the cytochrome c activation of caspase-3. Also associates with caspase-14, a short prodomain caspase. MW: 10000 and 19000.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Recommended reaction conditions
Activity Assay Required Materials • Assay Buffer: 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS • Substrate: 100 mM stock of appropriate substrate (e.g. Ac-LEHD-pNA, Cat. No. 218805), prepared in DMSO; diluted to 400 µM in Assay Buffer just prior to use (final concentration in the well = 200 µM) • Caspase-4: dilute to 2 U/µl just prior to use (final amount of enzyme per well = 30 U) • Spectrophotometer: capacity to measure at 405 nm • 1/2 Volume 96-Well Plate
Protocol: 1. Add 35 µl Assay Buffer to the desired number of wells in a 1/2 Volume 96-Well Plate; allow to equilibrate to 25°C. 2. Add 15 µl Caspase-4 (2 U/µl) to each well; include 2 blanks that contain 15 µl Assay Buffer in place of the enzyme. 3. To start the reaction, add 50 µl Substrate (400 µM in assay buffer); mix by pipetting up and down. 4. Continuously monitor the absorbance at 405 nm. 5. Data analysis: plot absorbance vs. time and determine the slope over the linear portion of the curve; convert change in absorbance/minute to substrate/minute using an extinction coefficient of 10,500 M-1cm-1 for pNA. Correct the values for a light path of ~0.5 cm and a 100 µl well of a 1/2 Volume Plate.
Purity≥90% by SDS-PAGE
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKamada, S., et al. 1997. Oncogene, 15, 285.
Nasir, J., et al. 1997. Mamm. Genome 8, 611.
Nath, R., et al. 1996. Biochem. J. 319, 683.