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12-323 Caspase 3 Fluorometric Substrate

Caspase 3 Fluorometric Substrate (Ac-Asp-Glu-Val-Asp-AMC) for use in Capase activity assays. Can be used to assay Apopain activity in vitro.

Caspase 3 Fluorometric Substrate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Activity Assay
12-323
5 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsDetection Methods
ACTFluorescent
Description
Catalogue Number12-323
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCaspase 3 Fluorometric Substrate
References
Product Information
Detection methodFluorescent
PresentationLyophilized powder.
Quality LevelMQ100
Applications
ApplicationCaspase 3 Fluorometric Substrate (Ac-Asp-Glu-Val-Asp-AMC) for use in Capase activity assays. Can be used to assay Apopain activity in vitro.
Key Applications
  • Activity Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Gene Symbol
  • CASP3
  • apopain
  • CASP-3
  • procaspase3
  • CPP32B
  • SCA-1
  • CPP-32
  • Apopain
  • CPP32
  • Yama
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in an Apopain activity assay as described in (Nicholson, D.W., et al., Nature 376: 37-43, 1995).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsLypholized: 2 years at -20°C. Rehydrated: 4 months at -20°C.
Packaging Information
Material Size5 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-323 04053252675539

Documentation

Caspase 3 Fluorometric Substrate SDS

Title

Safety Data Sheet (SDS) 

Caspase 3 Fluorometric Substrate Certificates of Analysis

TitleLot Number
Caspase 3 Fluorometric Substrate (Ac-Asp-Glu-Val-Asp-AMC) 2872209
Caspase 3 Fluorometric Substrate (Ac-Asp-Glu-Val-Asp-AMC) 2894687
Caspase 3 Fluorometric Substrate - 14521 14521
Caspase 3 Fluorometric Substrate - 18686 18686
Caspase 3 Fluorometric Substrate - 20549 20549
Caspase 3 Fluorometric Substrate - 23399 23399
Caspase 3 Fluorometric Substrate - 29881 29881
Caspase 3 Fluorometric Substrate - 31421 31421
Caspase 3 Fluorometric Substrate - 31858 31858

References

Reference overviewApplicationPub Med ID
Caspase activation is an early event in anthracycline-induced apoptosis and allows detection of apoptotic cells before they are ingested by phagocytes
Durrieu, F., et al
Exp Cell Res, 240:165-75 (1998)  1998

Kinase Assay9596989 9596989