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QIA70 Caspase-3 Activity Assay Kit, Fluorometric

Caspase-3 Activity Assay Kit, Fluorometric MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
QIA70
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityDetection Methods
A Broad Range Of SpeciesFluorometric
Description
Overview

This product has been discontinued.



Suitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02).
Catalogue NumberQIA70
Brand Family Calbiochem®
Materials Required but Not Delivered• 1 M dithiothreitol (DTT, Cleland regent) solution (1.54 g to 10 ml H2O) made fresh (can be stored at -20°C for one month).
• 2-20 µl, 20-200 µl and 200-1000 µl precision pipettes with disposable tips.
• Fluorescent plate reader capable of measuring excitation ~400 nm, and emission at ~505 nm.
• A centrifuge, for spinning tubes or 96-well plate and to pulse-spin the Recombinant Caspase-3 vials.
• Microcentrifuge tubes.
• Protease inhibitor cocktail set III (Cat. No. 539134)

Note: It is recommended to add protease inhibitor cocktail at a 1:500 dilution to Sample Buffer and Assay Buffer just before use. These protease inhibitors have been shown to exhibit no effect on recombinant caspase-3 activity.
References
ReferencesX. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
S. J. Martin and D. 1995 R. Green, Cell 82, 349.
Product Information
Detection methodFluorometric
Form96 Tests
Format96-well plate
Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
Quality LevelMQ100
Applications
Biological Information
Assay time2.5-3 h
Sample TypeCell lysates
Species Reactivity
  • A Broad Range Of Species
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36-23

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Do not breathe fumes.
Product Usage Statements
Intended useThe Calbiochem Caspase-3 Activity Assay is a non-isotopic enzyme assay for the in vitro detection of Caspase-3 activity in cell lysates. The assay can be adapted for 96-well plates for sensitive drug screening methods.
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Storage ConditionsUpon Receipt of the kit: Store Recombinant Caspase-3 (2 vials) at -80°C to maintain stability. Store all other kit components at -20°C. Once thawed, Caspase-3 Sample Buffer and Assay Buffer may be stored at 4°C. Do not expose reagents to excessive light.
Protect from Moisture Protect from moisture
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
Specifications
Global Trade Item Number
Catalogue Number GTIN
QIA70 0

Documentation

Caspase-3 Activity Assay Kit, Fluorometric SDS

Title

Safety Data Sheet (SDS) 

Caspase-3 Activity Assay Kit, Fluorometric Certificates of Analysis

TitleLot Number
QIA70

References

Reference overview
X. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
S. J. Martin and D. 1995 R. Green, Cell 82, 349.

Citations

Title
  • So-Young Park and Adriana Ferreira. (2005) The generation of a 17 kDa neurotoxic fragment: an alternative mechanism by which Tau mediates β-amyloid-induced neurodegeneration. Journal of Neuroscience 25, 5365-5375.
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