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208725 Calphostin C, Cladosporium cladosporioides - CAS 121263-19-2 - Calbiochem

A cell permeable, highly specific inhibitor of protein kinase C (IC₅₀ = 50 nM) that interacts with the protein's regulatory domain by competing at the binding site of diacylglycerol and phorbol esters.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: UCN-1028c

Primary Target: PKC
208725
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
121263-19-2C₄₄H₃₈O₁₄
Description
Overview

This product has been discontinued.



A cell permeable, highly specific inhibitor of protein kinase C (IC50 = 50 nM) that interacts with the protein’s regulatory domain by competing at the binding site of diacylglycerol and phorbol esters. Does not compete with Ca2+ or phospholipids. At higher concentrations inhibits myosin light chain kinase (IC50 > 5 µM), protein kinase A (IC50 > 50 µM), protein kinase G (IC50 > 25 µM), and p60v-src (IC50 > 50 µM). Induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Requires brief exposure to light for activation.
Catalogue Number208725
Brand Family Calbiochem®
SynonymsUCN-1028c
References
ReferencesJarvis, W.D., et al. 1994. Cancer Res. 54, 1707.
Svetlov, S., and Nigami, S. 1993. Biochim. Biophys. Acta 1177, 75.
Gopalakrishna, R., et al. 1992. FEBS Lett. 314, 149.
Shimamato, H., et al. 1992. Br. J. Pharmacol. 107, 282.
Bruns, R.F., et al. 1991. Biochem. Biophys. Res. Commun. 176, 288.
Tamaoki, T., et al. 1990. Biotechnology 8, 732.
Kobayashi, E., et al. 1989. Biochem. Biophys. Res. Commun. 159, 548.
Product Information
CAS number121263-19-2
ATP CompetitiveN
FormLight brown lyophilized solid
Hill FormulaC₄₄H₃₈O₁₄
Chemical formulaC₄₄H₃₈O₁₄
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetPKC
Primary Target IC<sub>50</sub>50 nM against protein kinase C
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208725 0

Documentation

Calphostin C, Cladosporium cladosporioides - CAS 121263-19-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Calphostin C, Cladosporium cladosporioides - CAS 121263-19-2 - Calbiochem Certificates of Analysis

TitleLot Number
208725

References

Reference overview
Jarvis, W.D., et al. 1994. Cancer Res. 54, 1707.
Svetlov, S., and Nigami, S. 1993. Biochim. Biophys. Acta 1177, 75.
Gopalakrishna, R., et al. 1992. FEBS Lett. 314, 149.
Shimamato, H., et al. 1992. Br. J. Pharmacol. 107, 282.
Bruns, R.F., et al. 1991. Biochem. Biophys. Res. Commun. 176, 288.
Tamaoki, T., et al. 1990. Biotechnology 8, 732.
Kobayashi, E., et al. 1989. Biochem. Biophys. Res. Commun. 159, 548.

Citations

Title
  • Martha E. O'Donnnell, et al. (2005) Arginine Vasopressin Stimulation of Cerebral Microvascular Endothelial Cell Na-K-Cl Cotransporter Activity is V1 Receptor and [Ca] Dependent. American Journal of Physiology Cell Physiology 289, C283-C292.
  • Yi Dai, et al. (2004) Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain. Journal of Neuroscience 24, 4293-4299.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision28-May-2008 RFH
    SynonymsUCN-1028c
    DescriptionCell-permeable, highly specific inhibitor of protein kinase C (IC50 = 50 nM) that interacts with the protein's regulatory domain by competing at the binding site of diacylglycerol and phorbol esters. At higher concentrations inhibits myosin light chain kinase (IC50 > 5 µM), protein kinase A (IC50 > 50 µM), protein kinase G (IC50 > 25 µM), and p60v-src protein tyrosine kinase (IC50 > 50 µM). Does not compete with Ca2+ or phospholipids. Also induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Inhibition of PKC is dependent on exposure to light in cell-free systems. Ordinary fluorescent light is sufficient for full activation.
    FormLight brown lyophilized solid
    CAS number121263-19-2
    Chemical formulaC₄₄H₃₈O₁₄
    Structure formulaStructure formula
    Purity≥95% by HPLC
    SolubilityDMSO (1 mg/ml) or Ethanol
    Storage Protect from light
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Standard Handling
    ReferencesJarvis, W.D., et al. 1994. Cancer Res. 54, 1707.
    Svetlov, S., and Nigami, S. 1993. Biochim. Biophys. Acta 1177, 75.
    Gopalakrishna, R., et al. 1992. FEBS Lett. 314, 149.
    Shimamato, H., et al. 1992. Br. J. Pharmacol. 107, 282.
    Bruns, R.F., et al. 1991. Biochem. Biophys. Res. Commun. 176, 288.
    Tamaoki, T., et al. 1990. Biotechnology 8, 732.
    Kobayashi, E., et al. 1989. Biochem. Biophys. Res. Commun. 159, 548.
    Citation
  • Martha E. O'Donnnell, et al. (2005) Arginine Vasopressin Stimulation of Cerebral Microvascular Endothelial Cell Na-K-Cl Cotransporter Activity is V1 Receptor and [Ca] Dependent. American Journal of Physiology Cell Physiology 289, C283-C292.
  • Yi Dai, et al. (2004) Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain. Journal of Neuroscience 24, 4293-4299.
    		•