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203350 Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem

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MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein synthesis
203350
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
3513-03-9C₁₇H₂₆N₈O5 · HCl

Products

Catalogue NumberPackaging Qty/Pack
203350-25MG Glass bottle 25 mg
Description
OverviewNucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for use as a dominant selectable marker in conjunction with blasticidin S resistant plasmids. Blasticidin S resistance is conferred by the blasticidin S deaminase gene (bcr), which converts blastisidin S to a nontoxic deaminohydroxy derivative.
Catalogue Number203350
Brand Family Calbiochem®
References
ReferencesKimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
Sutoh, K. 1993. Plasmid 30, 150.
Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.
Product Information
CAS number3513-03-9
FormWhite solid
Hill FormulaC₁₇H₂₆N₈O5 · HCl
Chemical formulaC₁₇H₂₆N₈O5 · HCl
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetprotein synthesis
Purity≥ 98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 28

Very toxic if swallowed.
S PhraseS: 24/25-36/37-45

Avoid contact with skin and eyes.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
203350-25MG 07790788048341

Documentation

Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Blasticidin S, Hydrochloride, Streptomyces griseochromogenes - CAS 589205 - Calbiochem Certificates of Analysis

TitleLot Number
203350

References

Reference overview
Kimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
Sutoh, K. 1993. Plasmid 30, 150.
Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.

Citations

Title
  • Richard L Bennett, et al. (2006) RAX, the PKR activator, sensitizes cells to inflammatory cytokines, serum withdrawal, chemotherapy and viral infection.. Blood in press,.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-September-2019 JSW
    DescriptionNucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus), whose products convert blasticidin S to a non-toxic deaminohydroxy derivative.
    FormWhite solid
    Recommended reaction conditionsTypically used at 2-10 µg/ml for mammalian cell selection.
    CAS number3513-03-9
    Chemical formulaC₁₇H₂₆N₈O5 · HCl
    Structure formulaStructure formula
    Purity≥ 98% by HPLC
    SolubilityH₂O (5 mg/ml) or Dilute Acetic Acid
    Storage +2°C to +8°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions (pH ≤7) are stable for up to 1 year at -20°C. Avoid alkaline pH. Do not subject to freeze/thaw cycles; upon thawing any aliquots, discard the unused portion.
    Toxicity Highly Toxic
    Merck USA index14, 1316
    ReferencesKimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653.
    Kimura, M., et al. 1994, Mol. Gen. Genet. 242, 121.
    Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451.
    Kudo, T., et al. 1994. J. Immunol. Methods 177, 17.
    Sutoh, K. 1993. Plasmid 30, 150.
    Izumi, M., et al. 1991. Exp. Cell Res. 197, 229.
    Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667.
    Takeuchi, S., et al. 1958. J. Antibiot. 11, 1.
    Citation
  • Richard L Bennett, et al. (2006) RAX, the PKR activator, sensitizes cells to inflammatory cytokines, serum withdrawal, chemotherapy and viral infection.. Blood in press,.
    		•