Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem

Cell-permeable, adenine-thymine-specific fluorescent stain.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342

382065
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
23491-52-3C₂₇H₂₈N₆O · 3HCl

Products

Catalogue NumberPackaging Qty/Pack
382065-100MG Alu drum 100 mg
Description
OverviewCell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
Catalogue Number382065
Brand Family Calbiochem®
Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
References
ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
Sandhu, L.C., et al. 1985. Cytometry 6, 191.
Frankfurt, O.S. 1983. Cytometry 4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
Visser, J.W.M. 1980. Flow Cytometry IV, 86.
Product Information
CAS number23491-52-3
ATP CompetitiveN
FormYellow solid
Hill FormulaC₂₇H₂₈N₆O · 3HCl
Chemical formulaC₂₇H₂₈N₆O · 3HCl
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetAdenine-thymine-specific fluorescent stain
Purity≥95% by TLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSDT4200000
Safety Information
R PhraseR: 23/24/25-36/37/38

Toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
382065-100MG 04055977213089

Documentation

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem Certificates of Analysis

TitleLot Number
382065

References

Reference overview
Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
Sandhu, L.C., et al. 1985. Cytometry 6, 191.
Frankfurt, O.S. 1983. Cytometry 4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
Visser, J.W.M. 1980. Flow Cytometry IV, 86.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-July-2008 RFH
Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
DescriptionCell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
FormYellow solid
CAS number23491-52-3
RTECSDT4200000
Chemical formulaC₂₇H₂₈N₆O · 3HCl
Structure formulaStructure formula
Purity≥95% by TLC
SolubilityH₂O (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
Toxicity Toxic & Carcinogenic / Teratogenic
ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
Sandhu, L.C., et al. 1985. Cytometry 6, 191.
Frankfurt, O.S. 1983. Cytometry 4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
Visser, J.W.M. 1980. Flow Cytometry IV, 86.